21 - 31 Thong KL.pmd
نویسندگان
چکیده
A PCR-based assay that can simultaneously detect and differentiate five different types of nosocomial bacterial pathogens was developed. Six pairs of selected primers targeting femA (132 bp) and mecA (310 bp) of methicillin-resistant Staphylococcus aureus, gltA (722 bp) of Acinetobacter baumannii, phoA (903 bp) of Escherichia coli, mdh (364 bp) of Klebsiella pneumoniae and oprL (504 bp) of Pseudomonas aeruginosa were used in this study. The conditions were optimized for the multiplex PCR to ensure specific amplification of the selected targets. Sensitivity and specificity tests were also carried out using a blind test approach on 50 bacterial cultures and resulted in 100% for both positive and negative predictive values.
منابع مشابه
689 - 697 Thong KL.pmd
The present study aims to develop a system which consists of four pairs of primers that specifically detects Salmonella spp., Salmonella serovar Typhi and Salmonella serovar Paratyphi A with an internal amplification control. The system, when applied in Polymerase Chain Reaction (PCR) under specific conditions, reaction mixture and cycling temperatures produced four bands; 784 bp, 496 bp, 332 b...
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INTRODUCTION Thong style flip-flop footwear and sandals are the preferred footwear of 22% of children in Australia [1]. However, little is known about the effects of thong wearing on the growing child, despite thongs being identified by parents as a primary contributor to children’s total foot complaints with 15% of forefoot and 22% of rearfoot foot complaints attributed to thong and sandal wea...
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A method to create an array of sub-5 nm nanogaps with self-aligned holes in a protective polymer overlayer is presented. The parallel formation of the nanogaps, intended for electrical sensing of biomolecules in an aqueous environment, is achieved by electromigration using a simple voltage ramp across parallel-connected electrode patterns with individual constrictions. It was observed that the ...
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The properties of Mon Thong, Chani and Kan Yao durian (Durio zibethinus Murr.) cultivars were compared in vitro and in vivo studies in order to find the best one as a supplement to antiatherosclerotic diet. Total polyphenols (361.4+/-35.3 mgGAE/100g FW), flavonoids (93.9+/-8.9 mgCE/100g FW) and total antioxidant capacity determined by DPPH and beta-carotene-linoleic acid assays (261.3+/-25.3 mi...
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Correspondence Kwai Lin Thong [email protected] Institute of Biological Science, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia Faculty of Agriculture and Biotechnology, Universiti Darul Iman Malaysia, 20400 Kuala Terengganu, Malaysia Specialized Diagnostic Centre, Institute of Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia Hospital Sultanah Aminah Johor Bh...
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